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Addgene

pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry
(Plasmid #225584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4TO
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    T21R-F8.2 (S54L+T127A)-T2A-mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    1452
  • Mutation
    T21R-F8.2 (S54L+T127A)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry was a gift from William McEwan (Addgene plasmid # 225584 ; http://n2t.net/addgene:225584 ; RRID:Addgene_225584)

  • For your References section:

    Aggregate-selective removal of pathological tau by clustering-activated degraders. Benn J, Cheng S, Keeling S, Smith AE, Vaysburd MJ, Boken D, Miller LVC, Katsinelos T, Franco C, Dupre E, Danis C, Landrieu I, Buee L, Klenerman D, James LC, McEwan WA. Science. 2024 Aug 30;385(6712):1009-1016. doi: 10.1126/science.adp5186. Epub 2024 Aug 29. 10.1126/science.adp5186 PubMed 39208111
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