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Addgene

hzHDAC10
(Plasmid #225542)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225542 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Backbone manufacturer
    Scott Gradia
  • Backbone size w/o insert (bp) 6465
  • Total vector size (bp) 8509
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Histone Deacetylase 10 humanized catalytic domain
  • Alt name
    HDAC10
  • Alt name
    hzHDAC10
  • Species
    D. rerio (zebrafish)
  • Mutation
    Mutated A24E and D94A
  • Entrez Gene
    hdac10 (a.k.a. wu:fd19e10, zgc:55652)
  • Promoter T7
  • Tag / Fusion Protein
    • His6-MBP tag with cleavable TEV site (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The original cloning was done by Dr. Corey J. Herbst-Gervasoni, a previous Christianson Lab member.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hzHDAC10 was a gift from David Christianson (Addgene plasmid # 225542 ; http://n2t.net/addgene:225542 ; RRID:Addgene_225542)

  • For your References section:

    Structural Basis for the Selective Inhibition of HDAC10, the Cytosolic Polyamine Deacetylase. Herbst-Gervasoni CJ, Steimbach RR, Morgen M, Miller AK, Christianson DW. ACS Chem Biol. 2020 Aug 21;15(8):2154-2163. doi: 10.1021/acschembio.0c00362. Epub 2020 Jul 23. 10.1021/acschembio.0c00362 PubMed 32659072
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