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Addgene

hCD12
(Plasmid #225541)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225541 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Backbone manufacturer
    Scott Gradia
  • Backbone size w/o insert (bp) 6465
  • Total vector size (bp) 8737
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Human Histone Deacetylase 6 Catalytic Domain 1 and 2
  • Alt name
    HDAC6
  • Alt name
    hCD12
  • Species
    H. sapiens (human)
  • Entrez Gene
    HDAC6 (a.k.a. CPBHM, HD6, JM21, KDAC6, PPP1R90)
  • Promoter T7
  • Tag / Fusion Protein
    • His6-MBP tag with cleavable TEV site (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The original cloning was done by Dr. Yang Hai, a previous Christianson Lab member.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hCD12 was a gift from David Christianson (Addgene plasmid # 225541 ; http://n2t.net/addgene:225541 ; RRID:Addgene_225541)

  • For your References section:

    Histone deacetylase 6 structure and molecular basis of catalysis and inhibition. Hai Y, Christianson DW. Nat Chem Biol. 2016 Sep;12(9):741-7. doi: 10.1038/nchembio.2134. Epub 2016 Jul 25. 10.1038/nchembio.2134 PubMed 27454933
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