pMH062
(Plasmid
#225537)
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PurposeExpresses IS621 in insect cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 225537 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFastBac
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIS621
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SpeciesEscherichia coli
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMH062 was a gift from Hiroshi Nishimasu (Addgene plasmid # 225537 ; http://n2t.net/addgene:225537 ; RRID:Addgene_225537) -
For your References section:
Structural mechanism of bridge RNA-guided recombination. Hiraizumi M, Perry NT, Durrant MG, Soma T, Nagahata N, Okazaki S, Athukoralage JS, Isayama Y, Pai JJ, Pawluk A, Konermann S, Yamashita K, Hsu PD, Nishimasu H. Nature. 2024 Jun;630(8018):994-1002. doi: 10.1038/s41586-024-07570-2. Epub 2024 Jun 26. 10.1038/s41586-024-07570-2 PubMed 38926616