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Addgene

H2B-iRFP
(Plasmid #225533)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piggyBac
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H2B
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.06.02.543474 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H2B-iRFP was a gift from Jared Toettcher (Addgene plasmid # 225533 ; http://n2t.net/addgene:225533 ; RRID:Addgene_225533)

  • For your References section:

    Recording morphogen signals reveals mechanisms underlying gastruloid symmetry breaking. McNamara HM, Solley SC, Adamson B, Chan MM, Toettcher JE. Nat Cell Biol. 2024 Oct 2. doi: 10.1038/s41556-024-01521-9. 10.1038/s41556-024-01521-9 PubMed 39358450
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