pHM039
(Plasmid
#225523)
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PurposeEncodes Wnt-responsive rtTA and constitutive BFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225523 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepiggyBac
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namertTA
Gene/Insert 2
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Gene/Insert nameBFP
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.06.02.543474 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHM039 was a gift from Jared Toettcher (Addgene plasmid # 225523 ; http://n2t.net/addgene:225523 ; RRID:Addgene_225523) -
For your References section:
Recording morphogen signals reveals mechanisms underlying gastruloid symmetry breaking. McNamara HM, Solley SC, Adamson B, Chan MM, Toettcher JE. Nat Cell Biol. 2024 Oct 2. doi: 10.1038/s41556-024-01521-9. 10.1038/s41556-024-01521-9 PubMed 39358450