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Flag-HA-OTUB1
(Plasmid #22551)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-N-Flag-HA-IRES-PURO
  • Backbone manufacturer
    Harper_Lab
  • Backbone size w/o insert (bp) 6521
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OTUB1
  • Alt name
    OTU domain, ubiquitin aldehyde binding 1
  • Alt name
    FLJ20113, FLJ40710, HSPC263, MGC111158, MGC4584, OTB1, OTU1
  • Species
    H. sapiens (human)
  • Mutation
    None
  • GenBank ID
    BC007519.2
  • Entrez Gene
    OTUB1 (a.k.a. HSPC263, OTB1, OTU1)
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site GATEWAY_LR (unknown if destroyed)
  • 3′ cloning site GATEWAY_LR (unknown if destroyed)
  • 5′ sequencing primer CAGCCCTCACTCCTTCTCTAGG
  • 3′ sequencing primer CAAGCGGCTTCGGCCAGTAAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Marc_Vidal_Orfeome_collection
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-HA-OTUB1 was a gift from Wade Harper (Addgene plasmid # 22551 ; http://n2t.net/addgene:22551 ; RRID:Addgene_22551)

  • For your References section:

    Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, Bennett EJ, Gygi SP, Harper JW. Cell. 2009 Jul 23. 138(2):389-403. 10.1016/j.cell.2009.04.042 PubMed 19615732
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