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pUb-G76V-GFP-IRES-DsRed
(Plasmid #225503)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225503 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2 DsRed-Express2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5262
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418) ; DsRed

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ub-G76V-GFP
  • Alt name
    Ubiquitin
  • Alt name
    Ub
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    990
  • Mutation
    Ubiquitin fused to N-terminus of GFP. Glycine 76 mutated to Valine.
  • Promoter CMV promoter
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-Forward
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Nico Dantuma, Addgene plasmid #11941

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was created by Dr Lidia Wrobel in the Rubinsztein lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUb-G76V-GFP-IRES-DsRed was a gift from David Rubinsztein (Addgene plasmid # 225503 ; http://n2t.net/addgene:225503 ; RRID:Addgene_225503)

  • For your References section:

    PSMC5 insufficiency and P320R mutation impair proteasome function. Yu ZQ, Carmichael J, Collins GA, D'Agostino MD, Lessard M, Firth HV, Harijan P, Fry AE, Dean J, Zhang J, Kini U, Goldberg AL, Rubinsztein DC. Hum Mol Genet. 2024 May 22:ddae085. doi: 10.1093/hmg/ddae085. 10.1093/hmg/ddae085 PubMed 38776958
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