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pCW57.1-p37-SHPmut-FLAG
(Plasmid #225502)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225502 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW57.1
  • Backbone size w/o insert (bp) 9354
  • Vector type
    Mammalian Expression, Lentiviral ; Gateway Destination vector, Doxycycline inducible
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p37
  • Alt name
    UBXN2B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    993
  • Mutation
    F215A/E218A/QKL220-222AAA
  • Entrez Gene
    UBXN2B (a.k.a. p37)
  • Promoter TRE promoter, Tet ON
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer LNCX
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was created by Dr Lidia Wrobel in the Rubinsztein lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57.1-p37-SHPmut-FLAG was a gift from David Rubinsztein (Addgene plasmid # 225502 ; http://n2t.net/addgene:225502 ; RRID:Addgene_225502)

  • For your References section:

    p37 regulates VCP/p97 shuttling and functions in the nucleus and cytosol. Wrobel L, Hoffmann JL, Li X, Rubinsztein DC. Sci Adv. 2024 May 3;10(18):eadl6082. doi: 10.1126/sciadv.adl6082. Epub 2024 May 3. 10.1126/sciadv.adl6082 PubMed 38701207
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