pEF-1α-p37-mClover2
(Plasmid
#225500)
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PurposeExpression of mClover2-tagged p37 protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225500 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSicoR (pMK1200)
- Backbone size w/o insert (bp) 7817
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Vector typeMammalian Expression, Lentiviral
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Selectable markersmClover2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namep37
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Alt nameUBXN2B
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SpeciesH. sapiens (human)
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Insert Size (bp)993
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Mutationno
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Entrez GeneUBXN2B (a.k.a. p37)
- Promoter EF1A
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Tag
/ Fusion Protein
- mClover2 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EF-1a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was created by Dr Lidia Wrobel in the Rubinsztein lab.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF-1α-p37-mClover2 was a gift from David Rubinsztein (Addgene plasmid # 225500 ; http://n2t.net/addgene:225500 ; RRID:Addgene_225500) -
For your References section:
p37 regulates VCP/p97 shuttling and functions in the nucleus and cytosol. Wrobel L, Hoffmann JL, Li X, Rubinsztein DC. Sci Adv. 2024 May 3;10(18):eadl6082. doi: 10.1126/sciadv.adl6082. Epub 2024 May 3. 10.1126/sciadv.adl6082 PubMed 38701207