pCMV5a-p37WT-FLAG
(Plasmid
#225498)
-
PurposeExpression of FLAG-tagged wildtype p37 protein
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 225498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFLAG-CMV-5a
-
Backbone manufacturerSigma-Aldrich
- Backbone size w/o insert (bp) 4664
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namep37
-
Alt nameUBXN2B
-
SpeciesH. sapiens (human)
-
Insert Size (bp)993
-
Mutationno
-
Entrez GeneUBXN2B (a.k.a. p37)
- Promoter CMV promoter
-
Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-Forward (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was created by Dr Lidia Wrobel in the Rubinsztein lab.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV5a-p37WT-FLAG was a gift from David Rubinsztein (Addgene plasmid # 225498 ; http://n2t.net/addgene:225498 ; RRID:Addgene_225498) -
For your References section:
p37 regulates VCP/p97 shuttling and functions in the nucleus and cytosol. Wrobel L, Hoffmann JL, Li X, Rubinsztein DC. Sci Adv. 2024 May 3;10(18):eadl6082. doi: 10.1126/sciadv.adl6082. Epub 2024 May 3. 10.1126/sciadv.adl6082 PubMed 38701207