pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP
(Plasmid
#225490)
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PurposeNeuronal expression vector for generating mTREK-1 (S131C/A286F) + IRES - GFP under the hSyn promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225490 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression ; Neuronal expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePotassium channel subfamily K member 2
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Alt nameKcnk2
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Alt nameTREK-1
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SpeciesM. musculus (mouse)
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Insert Size (bp)2625
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GenBank ID16526 NP_034737.2
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Entrez GeneKcnk2 (a.k.a. A430027H14Rik, TREK-1)
- Promoter hSyn
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP was a gift from Dan Minor (Addgene plasmid # 225490 ; http://n2t.net/addgene:225490 ; RRID:Addgene_225490) -
For your References section:
Development of covalent chemogenetic K(2P) channel activators. Deal PE, Lee H, Mondal A, Lolicato M, Mendonca PRF, Black H, Jang S, El-Hilali X, Bryant C, Isacoff EY, Renslo AR, Minor DL Jr. Cell Chem Biol. 2024 Jul 18;31(7):1305-1323.e9. doi: 10.1016/j.chembiol.2024.06.006. 10.1016/j.chembiol.2024.06.006 PubMed 39029456