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pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP
(Plasmid #225490)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression ; Neuronal expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Potassium channel subfamily K member 2
  • Alt name
    Kcnk2
  • Alt name
    TREK-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2625
  • GenBank ID
    16526 NP_034737.2
  • Entrez Gene
    Kcnk2 (a.k.a. A430027H14Rik, TREK-1)
  • Promoter hSyn

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NOTE: Also known as TREK-1CG* A286F and used in chemogenetic strategy termed CATKLAMP (covalent activation of TREK family K+ channels to clamp membrane potential) for irreversibly activating K2P potassium channels and thereby silencing of electrical activity in neurons in the presence of CAT335, a maleimide derivative of the K2P modulator pocket activator ML335.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP was a gift from Dan Minor (Addgene plasmid # 225490 ; http://n2t.net/addgene:225490 ; RRID:Addgene_225490)

  • For your References section:

    Development of covalent chemogenetic K(2P) channel activators. Deal PE, Lee H, Mondal A, Lolicato M, Mendonca PRF, Black H, Jang S, El-Hilali X, Bryant C, Isacoff EY, Renslo AR, Minor DL Jr. Cell Chem Biol. 2024 Jul 18;31(7):1305-1323.e9. doi: 10.1016/j.chembiol.2024.06.006. 10.1016/j.chembiol.2024.06.006 PubMed 39029456
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