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Addgene

pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB
(Plasmid #225488)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225488 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSIN-WP-mPGK-GDNF
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tetracycline-controlled transactivator fused with Krüppel-associated box (KRAB) domain following an internal ribosome entry site
  • Alt name
    IRES-tTA-KRAB
  • Alt name
    IRES-tTR-KRAB
  • Species
    Synthetic
  • Insert Size (bp)
    2935
  • Promoter Mouse phosphoglycerate kinase 1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer ccgctgagcaatggaagcg
  • 3′ sequencing primer gatttaggtgacactatag
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB was a gift from Robert Gross (Addgene plasmid # 225488 ; http://n2t.net/addgene:225488 ; RRID:Addgene_225488)

  • For your References section:

    C3 transferase gene therapy for continuous conditional RhoA inhibition. Gutekunst CA, Tung JK, McDougal ME, Gross RE. Neuroscience. 2016 Dec 17;339:308-318. doi: 10.1016/j.neuroscience.2016.10.022. Epub 2016 Oct 13. 10.1016/j.neuroscience.2016.10.022 PubMed 27746349
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