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Addgene

pET29b-rhMOG
(Plasmid #225484)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225484 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Backbone manufacturer
    MilliporeSigma (Novagen)
  • Backbone size w/o insert (bp) 5237
  • Total vector size (bp) 5618
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For expression, please use SHuffle® T7 Express lysY Competent E. coli (NEB)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Soluble Domain of Myelin Oligodendrocyte Glycoprotein
  • Alt name
    rhMOG
  • Alt name
    Myelin Oligodendrocyte Glycoprotein amino acids 30-149
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    384
  • Mutation
    deleted amino acids 1-29 (signal peptide) and 150-247 (transmembrane domain)
  • GenBank ID
    CAA52617.1
  • Entrez Gene
    MOG (a.k.a. BTN6, BTNL11, MOGIG2, NRCLP7)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis tag (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Twist Bioscience, South San Francisco, USA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.22.602974 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b-rhMOG was a gift from John Pak (Addgene plasmid # 225484 ; http://n2t.net/addgene:225484 ; RRID:Addgene_225484)

  • For your References section:

    High-yield production of recombinant human myelin oligodendrocyte glycoprotein in SHuffle bacteria without a refolding step. Wu W, Gupta S, Sagan SA, Moseley CE, Zamvil SS, Pak JE. J Immunol Methods. 2024 Oct 13:113764. doi: 10.1016/j.jim.2024.113764. 10.1016/j.jim.2024.113764 PubMed 39406335
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