LsgRNA-FXR1-KH1-gRNA
(Plasmid
#225483)
-
PurposeExpress gRNA for FXR1
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 225483 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneSpCas9 gRNA backbone
-
Backbone manufacturerCharles Gersbach (Addgene # 47108)
-
Vector typeCRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namegRNA for editing FXR1-KH1
-
gRNA/shRNA sequenceGTACCATGTGTTCCTATTGCC
-
SpeciesH. sapiens (human)
-
Entrez GeneFXR1 (a.k.a. CMYP9A, CMYP9B, FXR1P, MYOPMIL, MYORIBF)
- Promoter hU6
Cloning Information
- Cloning method Other
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.05.565677 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
LsgRNA-FXR1-KH1-gRNA was a gift from Christine Mayr (Addgene plasmid # 225483 ; http://n2t.net/addgene:225483 ; RRID:Addgene_225483) -
For your References section:
The FXR1 network acts as signaling scaffold for actomyosin remodeling. Chen X, Fansler MM, Janjoš U, Ule J, Mayr C. Cell. 5 August 2024. doi: 10.1016/j.cell.2024.07.015. 10.1016/j.cell.2024.07.015 PubMed 37961296