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Addgene

pU6-tevopreq1-FXR1-G266E
(Plasmid #225482)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225482 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SpCas9 epegRNA backbone
  • Backbone manufacturer
    David Liu (Addgene # 174038)
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    epegRNA for editing FXR1-G266E
  • gRNA/shRNA sequence
    GACTGGAACATTCAGAATCT
  • Species
    H. sapiens (human)
  • Entrez Gene
    FXR1 (a.k.a. CMYP9A, CMYP9B, FXR1P, MYOPMIL, MYORIBF)
  • Promoter hU6

Cloning Information

  • Cloning method Other

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.05.565677 for bioRxiv preprint.

Sequence of the FXR1-G266E prime editing epegRNA precursor:
Spacer - GACTGGAACATTCAGAATCT
Scaffold - AGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGC
Template - CTACGAAGAGGTAAGT
PBS - ATTCTGAATGTTC
Pseudoknot - cgcggttctatctagttacgcgttaaaccaactagaa

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-tevopreq1-FXR1-G266E was a gift from Christine Mayr (Addgene plasmid # 225482 ; http://n2t.net/addgene:225482 ; RRID:Addgene_225482)

  • For your References section:

    The FXR1 network acts as signaling scaffold for actomyosin remodeling. Chen X, Fansler MM, Janjoš U, Ule J, Mayr C. Cell. 5 August 2024. doi: 10.1016/j.cell.2024.07.015. 10.1016/j.cell.2024.07.015 PubMed 37961296
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