Anti-Cav1.2 Ca2+ channel [N263/31R-rat IgG2a]
(Plasmid
#225422)
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PurposeMammalian Expression Plasmid of anti-Cav1.2 Ca2+ channel (Rat) rat IgG2a R-mAb. Derived from hybridoma N263/31.
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Depositing Lab
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Sequence Information
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Sequences (3) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneP1316-rat IgG2a
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Backbone manufacturerGavin Wright, Sanger; Yves Durocher, NRCC; Giovanna Roncador,CNIO
- Backbone size w/o insert (bp) 5925
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnti-Cav1.2 Ca2+ channel (Rattus norvegicus) recombinant (Mouse) monoclonal antibody.
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SpeciesChimera: M. musculus (mouse)/R. norvegicus (rat)
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Entrez GeneCacna1c (a.k.a. RATIVS302)
- Promoter Dual CMV
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
RRID: AB_3112096. Isotype: rat IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology. Research Institute.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Anti-Cav1.2 Ca2+ channel [N263/31R-rat IgG2a] was a gift from James Trimmer (Addgene plasmid # 225422 ; http://n2t.net/addgene:225422 ; RRID:Addgene_225422) -
For your References section:
High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research. Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles ML, Froenicke L, Trimmer JS. Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4. 10.1038/s41598-023-43233-4 PubMed 37758930