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Anti-Kv2.1 K+ channel [K89/34R-rat IgG2a]
(Plasmid #225417)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225417 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    P1316-rat IgG2a
  • Backbone manufacturer
    Gavin Wright, Sanger; Yves Durocher, NRCC; Giovanna Roncador,CNIO
  • Backbone size w/o insert (bp) 5925
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Anti-Kv2.1 K+ channel (Rattus norvegicus) recombinant (Mouse) monoclonal antibody.
  • Species
    Chimera: M. musculus (mouse)/R. norvegicus (rat)
  • Entrez Gene
    Kcnb1 (a.k.a. DRK1PC, Kcr1-1, Kv2.1, Shab)
  • Promoter Dual CMV

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

RRID: AB_3112087. Isotype: rat IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology. Research Institute.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-Kv2.1 K+ channel [K89/34R-rat IgG2a] was a gift from James Trimmer (Addgene plasmid # 225417 ; http://n2t.net/addgene:225417 ; RRID:Addgene_225417)

  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360
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