Skip to main content
Addgene

aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA
(Plasmid #225350)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225350 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    MTK0-002
  • Backbone manufacturer
    Plasmid #123960 El_Samad Lab
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    H2B
  • Tags / Fusion Proteins
    • non-fluorescent GFP (Y67F)-P2A (N terminal on insert)
    • miRFP (C terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    MTK0-002 piggyback backbone from EL_Samad lab Mammalian Cloning Toolkit (MTK) (Fonseca et al., 2019) pCAG promoter from MTK2-005 EL_Samad lab Mammalian Cloning Toolkit (MTK) (Fonseca et al., 2019)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

P2A-H2B-IRFP670 synthesized as a gblock

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA was a gift from Wendell Lim (Addgene plasmid # 225350 ; http://n2t.net/addgene:225350 ; RRID:Addgene_225350)
Related items: