pCAG-hHIF1 alpha-IGd2
(Plasmid
#22535)
-
PurposeTo be used for the RNAi screening purposes (not for HDAC4 expression); see paper
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22535 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCAG IGd2
- Backbone size w/o insert (bp) 6262
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namehypoxia inducible factor 1 alpha
-
SpeciesH. sapiens (human)
-
Insert Size (bp)3200
-
GenBank IDACN88547
-
Entrez GeneHIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ECORV (destroyed during cloning)
- 3′ cloning site ECORV (destroyed during cloning)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCAG-hHIF1 alpha-IGd2 was a gift from Connie Cepko (Addgene plasmid # 22535 ; http://n2t.net/addgene:22535 ; RRID:Addgene_22535) -
For your References section:
HDAC4 regulates neuronal survival in normal and diseased retinas. Chen B, Cepko CL. Science. 2009 Jan 9. 323(5911):256-9. 10.1126/science.1166226 PubMed 19131628