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Kif1bβ-pcDNA3
(Plasmid #22534)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22534 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5362
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha, XL1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kif1bβ
  • Alt name
    kinesin family member 1B (KIF1B), transcript variant 1 (beta)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5313
  • Mutation
    wt cDNA
  • GenBank ID
    NM_015074
  • Entrez Gene
    KIF1B (a.k.a. CMT2, CMT2A, CMT2A1, HMSNII, KLP, NBLST1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer T7 or CMV-forward
  • 3′ sequencing primer SP6 or BGH-reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Kif1bβ-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 22534 ; http://n2t.net/addgene:22534 ; RRID:Addgene_22534)

  • For your References section:

    The kinesin KIF1Bbeta acts downstream from EglN3 to induce apoptosis and is a potential 1p36 tumor suppressor. Schlisio S, Kenchappa RS, Vredeveld LC, George RE, Stewart R, Greulich H, Shahriari K, Nguyen NV, Pigny P, Dahia PL, Pomeroy SL, Maris JM, Look AT, Meyerson M, Peeper DS, Carter BD, Kaelin WG. Genes Dev. 2008 Apr 1. 22(7):884-93. 10.1101/gad.1648608 PubMed 18334619
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