pAc-Neo-OVA
(Plasmid #22533)

• Depositing Lab: Michael Bevan
• Publication: Moore et al Cell. 1988 Sep 9. 54(6):777-85.

Backbone

• Vector backbone: pHBetaAPr-1-neo
• Backbone manufacturer: Kedes Lab
• Backbone size w/o insert (bp): 10200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: OVA
• Species: G. gallus (chicken)
• Insert Size (bp): 1700
• Entrez Gene: OVAL (a.k.a. OVA, SERPINB14)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. B.W. O'Malley
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The OVA cDNA was removed from pOVA(230) (McReynolds et al., 1978) by digestion with EcoRI and subcloned into the polylinker of pUG-1. This provided convenient BglII and HindIII restriction sites adjacent to the ovalbumin cDNA for direct subcloning into the BamHI-HindIII site of the pHBetaAPr-1-neo express vector.