pXD80EN1-C1858_02810-(–)-Eldh2-noAccs (ElA2)
(Plasmid
#225309)
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PurposeExpresses Eggerthella lenta A2 (–)-Eldh2 without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225309 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepXD68Kan2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name(–)-Eldh2
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Alt name(–)-dihydroxyenterolactone dehydroxylase 2
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Alt nameC1858_02810
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SpeciesEggerthella lenta
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXD80EN1-C1858_02810-(–)-Eldh2-noAccs (ElA2) was a gift from Emily Balskus (Addgene plasmid # 225309 ; http://n2t.net/addgene:225309 ; RRID:Addgene_225309) -
For your References section:
Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary Polyphenols. Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP. J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 10.1021/jacs.4c09892. Epub 2025 Feb 24. 10.1021/jacs.4c09892 PubMed 39993729