pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378)
(Plasmid
#225305)
-
PurposeExpresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 and (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 225305 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepXD69m2
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert name(+)-Eldh1, (+)-Eldh2
-
Alt name(+)-dihydroxyenterolactone dehydroxylase 1, (+)-dihydroxyenterolactone dehydroxylase 2
-
Alt nameGPA_25810, GPA_25800
-
SpeciesGordonibacter pamelaeae DSM 19378
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378) was a gift from Emily Balskus (Addgene plasmid # 225305 ; http://n2t.net/addgene:225305 ; RRID:Addgene_225305) -
For your References section:
Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary Polyphenols. Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP. J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 10.1021/jacs.4c09892. Epub 2025 Feb 24. 10.1021/jacs.4c09892 PubMed 39993729