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pLenti X2 Zeo/pTER shGFP #2 (790-1)
(Plasmid #22529)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22529 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size w/o insert (bp) 5597
  • Vector type
    Lentiviral, RNAi
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3, 37oC.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sh Enhanced Green Fluorescent Protein #2
  • Alt name
    shGFP #2
  • Insert Size (bp)
    48

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (destroyed during cloning)
  • 3′ cloning site Hind III (destroyed during cloning)
  • 5′ sequencing primer H1forw
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti X2 Zeo/pTER shGFP #2 (790-1) was a gift from Eric Campeau (Addgene plasmid # 22529 ; http://n2t.net/addgene:22529 ; RRID:Addgene_22529)
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