pENTR/pTER shATM #2 (730-2)
(Plasmid
#22527)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR1A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2571
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Vector typeRNAi ; Entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10F', 37oC
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesh Ataxia telangiectasia mutated
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Alt nameshATM
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Insert Size (bp)48
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (destroyed during cloning)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer ENTRforw
- 3′ sequencing primer ENTRrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
shATM #2 was used successfully in Rodier et al.(2009) Nat.Cell Biol. 11(8):973-979.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR/pTER shATM #2 (730-2) was a gift from Eric Campeau (Addgene plasmid # 22527 ; http://n2t.net/addgene:22527 ; RRID:Addgene_22527)