pRSETA-qmTQ2-cAMP
(Plasmid
#225249)
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PurposeBacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSETA
- Backbone size w/o insert (bp) 2897
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameqmTQ2-cAMP
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SpeciesSynthetic
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Insert Size (bp)1218
- Promoter T7 promoter
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Tag
/ Fusion Protein
- 6XHis, T7 tag, XpressTM tag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSETA-qmTQ2-cAMP was a gift from Yasushi Okada (Addgene plasmid # 225249 ; http://n2t.net/addgene:225249 ; RRID:Addgene_225249) -
For your References section:
Development of fluorescence lifetime biosensors for ATP, cAMP, citrate, and glucose using the mTurquoise2-based platform. Zhong C, Arai S, Okada Y. Cell Rep Methods. 2024 Nov 18;4(11):100902. doi: 10.1016/j.crmeth.2024.100902. 10.1016/j.crmeth.2024.100902 PubMed 39561716