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Addgene

pcDNA3.1(-)-qmTQ2-ATP
(Plasmid #225248)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225248 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone size w/o insert (bp) 5427
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    qmTQ2-ATP
  • Species
    Synthetic
  • Insert Size (bp)
    1137
  • Promoter CMV promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(-)-qmTQ2-ATP was a gift from Yasushi Okada (Addgene plasmid # 225248 ; http://n2t.net/addgene:225248 ; RRID:Addgene_225248)
  • For your References section:

    Development of fluorescence lifetime biosensors for ATP, cAMP, citrate, and glucose using the mTurquoise2-based platform. Zhong C, Arai S, Okada Y. Cell Rep Methods. 2024 Nov 18;4(11):100902. doi: 10.1016/j.crmeth.2024.100902. 10.1016/j.crmeth.2024.100902 PubMed 39561716