pcDNA3.1(-)-qmTQ2-ATP
(Plasmid
#225248)
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Purposemammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225248 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(-)
- Backbone size w/o insert (bp) 5427
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameqmTQ2-ATP
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SpeciesSynthetic
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Insert Size (bp)1137
- Promoter CMV promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(-)-qmTQ2-ATP was a gift from Yasushi Okada (Addgene plasmid # 225248 ; http://n2t.net/addgene:225248 ; RRID:Addgene_225248) -
For your References section:
Development of fluorescence lifetime biosensors for ATP, cAMP, citrate, and glucose using the mTurquoise2-based platform. Zhong C, Arai S, Okada Y. Cell Rep Methods. 2024 Nov 18;4(11):100902. doi: 10.1016/j.crmeth.2024.100902. 10.1016/j.crmeth.2024.100902 PubMed 39561716