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Addgene

pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG
(Plasmid #225245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iPASnFR-H
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • mScarlet3 (C terminal on insert)
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.08.21.609037 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG was a gift from Loren Looger (Addgene plasmid # 225245 ; http://n2t.net/addgene:225245 ; RRID:Addgene_225245)

  • For your References section:

    Genetically encoded fluorescent sensors for visualizing polyamine levels, uptake, and distribution. Tamura R, Chen J, De Jaeger M, Morris JF, Scott DA, Vangheluwe P, Looger LL. bioRxiv 2024.08.21.609037 10.1101/2024.08.21.609037
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