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pEF-ENTR-LUC (SN1)
(Plasmid #22524)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22524 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR1A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4058
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10F', 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Firefly Luciferase
  • Insert Size (bp)
    1652

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RV (destroyed during cloning)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer T7forw
  • 3′ sequencing primer ENTRrev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Firefly luciferase was derived from pGL3 plasmid (Promega). EF1a promoter was derived from pTracer-Bsd plasmid (Invitrogen).
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Vector made by Stefania Nicoli (University of Massachusetts Medical School).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF-ENTR-LUC (SN1) was a gift from Eric Campeau (Addgene plasmid # 22524 ; http://n2t.net/addgene:22524 ; RRID:Addgene_22524)
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