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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22523 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR1A
- Backbone size w/o insert (bp) 2295
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Vector typeEntry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10F'
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDiscosoma sp. red fluorescent protein 2
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Alt nameDsRed2
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Insert Size (bp)677
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Not I (not destroyed)
- 5′ sequencing primer ENTRforw
- 3′ sequencing primer ENTRrev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDsRed2 cDNA obtained from Dr. Christian Beausejour.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-DsRed2 N1 (CMB1) was a gift from Eric Campeau (Addgene plasmid # 22523 ; http://n2t.net/addgene:22523 ; RRID:Addgene_22523)