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Addgene

pETcon-pGAL1-Aga2-Htt(25Q)-c_myc
(Plasmid #225228)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225228 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETcon(-)
  • Backbone size w/o insert (bp) 5740
  • Total vector size (bp) 6415
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Aga2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    261
  • GenBank ID
    852851
  • Tag / Fusion Protein
    • HA Tag (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Htt (25Q)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    276
  • GenBank ID
    3064
  • Tag / Fusion Protein
    • c-myc Tag (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETcon-pGAL1-Aga2-Htt(25Q)-c_myc was a gift from Urartu Seker (Addgene plasmid # 225228 ; http://n2t.net/addgene:225228 ; RRID:Addgene_225228)

  • For your References section:

    Highly Potent Peptide Therapeutics To Prevent Protein Aggregation in Huntington's Disease. Khan A, Ozcelik CE, Begli O, Oguz O, Kesici MS, Kasirga TS, Ozcubukcu S, Yuca E, Seker UOS. ACS Med Chem Lett. 2023 Nov 14;14(12):1821-1826. doi: 10.1021/acsmedchemlett.3c00415. eCollection 2023 Dec 14. 10.1021/acsmedchemlett.3c00415 PubMed 38116434
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