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Addgene

MYC-hTRAK1
(Plasmid #225204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag3A-myc vector
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 4333
  • Total vector size (bp) 7198
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    trak1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2910
  • Entrez Gene
    TRAK1 (a.k.a. DEE68, EIEE68, MILT1, OIP106)
  • Promoter CMV
  • Tag / Fusion Protein
    • MYC (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site bamhI (unknown if destroyed)
  • 3′ cloning site xhoI (unknown if destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Schwarz lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MYC-hTRAK1 was a gift from Thomas Schwarz (Addgene plasmid # 225204 ; http://n2t.net/addgene:225204 ; RRID:Addgene_225204)

  • For your References section:

    Glucose regulates mitochondrial motility via Milton modification by O-GlcNAc transferase. Pekkurnaz G, Trinidad JC, Wang X, Kong D, Schwarz TL. Cell. 2014 Jul 3;158(1):54-68. doi: 10.1016/j.cell.2014.06.007. 10.1016/j.cell.2014.06.007 PubMed 24995978
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