pAX1_HS∆P2
(Plasmid
#225197)
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PurposeUsed to create a markerless deletion of the E. coli HS P2 phage
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225197 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAX1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, Gentamicin and Chloramphenicol
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameP2 phage homology arms
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SpeciesEscherichia coli
Cloning Information
- Cloning method Other
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid is temperature sensitive and lost when grown at 37°C.
Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAX1_HS∆P2 was a gift from Travis Wiles (Addgene plasmid # 225197 ; http://n2t.net/addgene:225197 ; RRID:Addgene_225197) -
For your References section:
Phollow reveals in situ phage transmission dynamics in the zebrafish gut microbiome at single-virion resolution. Ortiz de Ora L, Wiles ET, Zund M, Banuelos MS, Haro-Ramirez N, Suder DS, Ujagar N, Ayala-Angulo J, Trinh C, Knitter C, Gonen S, Nicholas DA, Wiles TJ. Nat Microbiol. 2025 Apr 18. doi: 10.1038/s41564-025-01981-1. 10.1038/s41564-025-01981-1 PubMed 40251446