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Addgene

pTn7xTS_SpyCatcher:mTFP1
(Plasmid #225189)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225189 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTn7xTS
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Gentamicin, 100 & 10 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyCatcher:mTFP1
  • Species
    Other
  • Tag / Fusion Protein
    • SpyCatcher (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid is temperature sensitive and lost when grown at 37°C.

Please visit https://doi.org/10.1101/2024.06.12.598711 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTn7xTS_SpyCatcher:mTFP1 was a gift from Travis Wiles (Addgene plasmid # 225189 ; http://n2t.net/addgene:225189 ; RRID:Addgene_225189)

  • For your References section:

    Phollow reveals in situ phage transmission dynamics in the zebrafish gut microbiome at single-virion resolution. Ortiz de Ora L, Wiles ET, Zund M, Banuelos MS, Haro-Ramirez N, Suder DS, Ujagar N, Ayala-Angulo J, Trinh C, Knitter C, Gonen S, Nicholas DA, Wiles TJ. Nat Microbiol. 2025 Apr 18. doi: 10.1038/s41564-025-01981-1. 10.1038/s41564-025-01981-1 PubMed 40251446
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