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Addgene

pHBJT034
(Plasmid #225180)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225180 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHBJT023
  • Backbone size (bp) 5007
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Codon optimized mCherry sequence was synthesized by Twist Biosciences

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pHBJT023 containing IPTG-inducible mCherry

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHBJT034 was a gift from Jenny-Lee Thomassin (Addgene plasmid # 225180 ; http://n2t.net/addgene:225180 ; RRID:Addgene_225180)
  • For your References section:

    Generation of a plasmid series for rapid sub-cloning and use in various Enterobacteriaceae. Braun HG, Kanwal N, Rivera Lopez LF, Thomassin JL. J Biosci Bioeng. 2024 Sep 6:S1389-1723(24)00253-6. doi: 10.1016/j.jbiosc.2024.08.006. 10.1016/j.jbiosc.2024.08.006 PubMed 39244484