ph2m
(Plasmid #22518)

• Depositing Lab: Christopher Buck
• Publication: Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

Backbone

• Vector backbone: phGf
• Backbone size w/o insert (bp): 3975
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MCPyV VP2
• Alt name: MCV
• Alt name: Merkel Cell Polyomavirus
• Alt name: codon modified
• Species: Merkel Cell Polyomavirus
• Insert Size (bp): 725
• Mutation: codon modified
• GenBank ID: FJ548569

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attR1 (destroyed during cloning)
• 3′ cloning site: attR2 (destroyed during cloning)
• 5′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

First reference to this plasmid was in:

Human Merkel cell polyomavirus infection II. MCV is a common human infection that can be detected by conformational capsid epitope immunoassays. Tolstov YL, Pastrana DV, Feng H, Becker JC, Jenkins FJ, Moschos S, Chang Y, Buck CB, Moore PS. Int J Cancer. 2009 Sep 15.125(6):1250-6

Genbank style annotated sequences can be found at:
http://home.ccr.cancer.gov/LCO/packaging.htm