pHBJT031
(Plasmid
#225177)
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Purpose(Empty Backbone) Low copy cloning vector with mCherry integrated in the multiple cloning site (AmpR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 225177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHBJT017
- Backbone size (bp) 4228
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Vector typeBacterial Expression, Synthetic Biology
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCodon optimized mCherry sequence was synthesized by Twist Biosciences
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pHBJT017 containing IPTG-inducible mCherry
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHBJT031 was a gift from Jenny-Lee Thomassin (Addgene plasmid # 225177 ; http://n2t.net/addgene:225177 ; RRID:Addgene_225177) -
For your References section:
Generation of a plasmid series for rapid sub-cloning and use in various Enterobacteriaceae. Braun HG, Kanwal N, Rivera Lopez LF, Thomassin JL. J Biosci Bioeng. 2024 Sep 6:S1389-1723(24)00253-6. doi: 10.1016/j.jbiosc.2024.08.006. 10.1016/j.jbiosc.2024.08.006 PubMed 39244484
