pHBJT016
(Plasmid
#225167)
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Purpose(Empty Backbone) Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (ChlR).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225167 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
- Backbone size (bp) 4024
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Low copy plasmid (pSC101 ori and repA), pUC18 MCS, confers chloramphenicol resistance
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHBJT016 was a gift from Jenny-Lee Thomassin (Addgene plasmid # 225167 ; http://n2t.net/addgene:225167 ; RRID:Addgene_225167) -
For your References section:
Generation of a plasmid series for rapid sub-cloning and use in various Enterobacteriaceae. Braun HG, Kanwal N, Rivera Lopez LF, Thomassin JL. J Biosci Bioeng. 2024 Sep 6:S1389-1723(24)00253-6. doi: 10.1016/j.jbiosc.2024.08.006. 10.1016/j.jbiosc.2024.08.006 PubMed 39244484