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Addgene

pHBJT012
(Plasmid #225163)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225163 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Backbone size (bp) 3662
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Tag / Fusion Protein
    • FLAG epitope tag (35 bp) (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • FLAG epitope tag (35 bp) (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Low copy plasmid (pSC101 ori and repA), pUC18 MCS with FLAG epitope integrated at SmaI site, confers gentamycin resistance

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHBJT012 was a gift from Jenny-Lee Thomassin (Addgene plasmid # 225163 ; http://n2t.net/addgene:225163 ; RRID:Addgene_225163)

  • For your References section:

    Generation of a plasmid series for rapid sub-cloning and use in various Enterobacteriaceae. Braun HG, Kanwal N, Rivera Lopez LF, Thomassin JL. J Biosci Bioeng. 2024 Sep 6:S1389-1723(24)00253-6. doi: 10.1016/j.jbiosc.2024.08.006. 10.1016/j.jbiosc.2024.08.006 PubMed 39244484
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