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Addgene

MYC-hTRAK2
(Plasmid #225142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag3A-myc
  • Backbone manufacturer
    Agilent Technologies
  • Total vector size (bp) 7029
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRAK2
  • Alt name
    GRIF-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2760
  • Entrez Gene
    TRAK2 (a.k.a. ALS2CR3, CALS-C, GRIF-1, GRIF1, MILT2, OIP98)
  • Promoter CMV
  • Tag / Fusion Protein
    • MYC (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGGGCGGATCCAATGAGTCAATCCCAGAATGCA
  • 3′ sequencing primer CCCCCTCGAGACTCAGTCCTCCTTCAGGACACC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Previously published Schwarz lab plasmid.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: primers listed under 5' and 3' sequencing primers are those used to amplify TRAK2 with overhangs to do Gibson cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MYC-hTRAK2 was a gift from Thomas Schwarz (Addgene plasmid # 225142 ; http://n2t.net/addgene:225142 ; RRID:Addgene_225142)

  • For your References section:

    Miro GTPase domains regulate the assembly of the mitochondrial motor-adaptor complex. Davis K, Basu H, Izquierdo-Villalba I, Shurberg E, Schwarz TL. Life Sci Alliance. 2022 Oct 27;6(1):e202201406. doi: 10.26508/lsa.202201406. Print 2023 Jan. 10.26508/lsa.202201406 PubMed 36302649
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