pEMG-GRIS
(Plasmid
#225134)
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Purpose(Empty Backbone) For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 225134 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepEMG-GRIS
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. coli PIR2
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Copy numberLow Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMG-GRIS was a gift from Lars Blank (Addgene plasmid # 225134 ; http://n2t.net/addgene:225134 ; RRID:Addgene_225134) -
For your References section:
Establishing a straightforward I-SceI-mediated recombination one-plasmid system for efficient genome editing in P. putida KT2440. Meng H, Kobbing S, Blank LM. Microb Biotechnol. 2024 Jul;17(7):e14531. doi: 10.1111/1751-7915.14531. 10.1111/1751-7915.14531 PubMed 39031514
Map uploaded by the depositor.
