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Addgene

pET29b(+)_gp1(A11V)
(Plasmid #225131)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225131 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET-29(+)
  • Backbone manufacturer
    Twist Bioscience
  • Total vector size (bp) 5536
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gp1 (A11V)
  • Species
    bacteriophage GIL01
  • Mutation
    Alanine 11 to Valine
  • GenBank ID
    AJ536073.2
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His-tag (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Twist Bioscience

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b(+)_gp1(A11V) was a gift from Matej Butala (Addgene plasmid # 225131 ; http://n2t.net/addgene:225131 ; RRID:Addgene_225131)