pET29b(+)_gp1(A11V)
(Plasmid
#225131)
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PurposePhage GIL01 protein gp1 (A11V) mutant with a single nucleotide substitution (C32T)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225131 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-29(+)
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Backbone manufacturerTwist Bioscience
- Total vector size (bp) 5536
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegp1 (A11V)
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Speciesbacteriophage GIL01
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MutationAlanine 11 to Valine
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GenBank IDAJ536073.2
- Promoter T7 promoter
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Tag
/ Fusion Protein
- His-tag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 forward (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byTwist Bioscience
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET29b(+)_gp1(A11V) was a gift from Matej Butala (Addgene plasmid # 225131 ; http://n2t.net/addgene:225131 ; RRID:Addgene_225131)