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Addgene

pP4-C
(Plasmid #225097)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225097 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4615
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    synthetic riboswitch
  • Species
    Bacillus subtilis
  • Insert Size (bp)
    200
  • Promoter synthetic constitutive promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NciI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GCGCTAGCCACAGCTAACAC
  • 3′ sequencing primer GTGTTGGCCATGGAACAGGTAGTTTTCCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pP4-C was a gift from Robert Batey (Addgene plasmid # 225097 ; http://n2t.net/addgene:225097 ; RRID:Addgene_225097)

  • For your References section:

    Requirements for efficient ligand-gated co-transcriptional switching in designed variants of the B. subtilis pbuE adenine-responsive riboswitch in E. coli. Drogalis LK, Batey RT. PLoS One. 2020 Dec 1;15(12):e0243155. doi: 10.1371/journal.pone.0243155. eCollection 2020. 10.1371/journal.pone.0243155 PubMed 33259551
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