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Addgene

TadCBE-Cas9NG
(Plasmid #225093)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225093 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pD881-SR
  • Backbone manufacturer
    Atum
  • Total vector size (bp) 7650
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Use BL21 Star DE3 competent cells and rhamnose induction for protein purification
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TadCBE-Cas9NG
  • Species
    Synthetic
  • Tag / Fusion Protein
    • 8xHis (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    David Liu lab (Broad Institute)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.09.09.611392 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TadCBE-Cas9NG was a gift from Vijay Sankaran (Addgene plasmid # 225093 ; http://n2t.net/addgene:225093 ; RRID:Addgene_225093)

  • For your References section:

    Transcription factor networks disproportionately enrich for heritability of blood cell phenotypes. Martin-Rufino JD, Caulier A, Lee S, Castano N, King E, Joubran S, Jones M, Goldman SR, Arora UP, Wahlster L, Lander ES, Sankaran VG. bioRxiv 2024.09.09.611392 10.1101/2024.09.09.611392
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