pET28a:ChrH
(Plasmid
#225084)
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PurposeExpresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225084 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChrH
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SpeciesChryseobacterium sp. JM1
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Insert Size (bp)1152
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MutationWT
- Promoter T7
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Tag
/ Fusion Protein
- His6-Thrombin (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a:ChrH was a gift from Wilfred van der Donk (Addgene plasmid # 225084 ; http://n2t.net/addgene:225084 ; RRID:Addgene_225084) -
For your References section:
Macrocyclization and Backbone Rearrangement During RiPP Biosynthesis by a SAM-Dependent Domain-of-Unknown-Function 692. Ayikpoe RS, Zhu L, Chen JY, Ting CP, van der Donk WA. ACS Cent Sci. 2023 Apr 24;9(5):1008-1018. doi: 10.1021/acscentsci.3c00160. eCollection 2023 May 24. 10.1021/acscentsci.3c00160 PubMed 37252350