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Addgene

pACYCDuet-1:ChrA-C63S
(Plasmid #225080)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225080 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYCDuet-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ChrA-C63S
  • Species
    Chryseobacterium sp. JM1
  • Insert Size (bp)
    246
  • Mutation
    C63S
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACYCDuet-1:ChrA-C63S was a gift from Wilfred van der Donk (Addgene plasmid # 225080 ; http://n2t.net/addgene:225080 ; RRID:Addgene_225080)

  • For your References section:

    Macrocyclization and Backbone Rearrangement During RiPP Biosynthesis by a SAM-Dependent Domain-of-Unknown-Function 692. Ayikpoe RS, Zhu L, Chen JY, Ting CP, van der Donk WA. ACS Cent Sci. 2023 Apr 24;9(5):1008-1018. doi: 10.1021/acscentsci.3c00160. eCollection 2023 May 24. 10.1021/acscentsci.3c00160 PubMed 37252350
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