pET28:LanIV-Chondromyces
(Plasmid
#225078)
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PurposeExpresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLanA (LanIV-Chondromyces)
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SpeciesChondromyces crocatus sp. Cm c5
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Insert Size (bp)198
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MutationWT
- Promoter T7
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Tag
/ Fusion Protein
- His6 (N terminal on insert)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also encodes LanL (LanIV-Chondromyces)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28:LanIV-Chondromyces was a gift from Wilfred van der Donk (Addgene plasmid # 225078 ; http://n2t.net/addgene:225078 ; RRID:Addgene_225078) -
For your References section:
A scalable platform to discover antimicrobials of ribosomal origin. Ayikpoe RS, Shi C, Battiste AJ, Eslami SM, Ramesh S, Simon MA, Bothwell IR, Lee H, Rice AJ, Ren H, Tian Q, Harris LA, Sarksian R, Zhu L, Frerk AM, Precord TW, van der Donk WA, Mitchell DA, Zhao H. Nat Commun. 2022 Oct 17;13(1):6135. doi: 10.1038/s41467-022-33890-w. 10.1038/s41467-022-33890-w PubMed 36253467