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Addgene

pET28:LanII-57
(Plasmid #225074)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225074 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LanA(A) (LanII-57)
  • Species
    Kitasatospora xanthocidica
  • Insert Size (bp)
    216
  • Mutation
    WT
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also encodes His6-tagged LanA(B) (LanII-57) and LanM (LanII-57)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28:LanII-57 was a gift from Wilfred van der Donk (Addgene plasmid # 225074 ; http://n2t.net/addgene:225074 ; RRID:Addgene_225074)

  • For your References section:

    A scalable platform to discover antimicrobials of ribosomal origin. Ayikpoe RS, Shi C, Battiste AJ, Eslami SM, Ramesh S, Simon MA, Bothwell IR, Lee H, Rice AJ, Ren H, Tian Q, Harris LA, Sarksian R, Zhu L, Frerk AM, Precord TW, van der Donk WA, Mitchell DA, Zhao H. Nat Commun. 2022 Oct 17;13(1):6135. doi: 10.1038/s41467-022-33890-w. 10.1038/s41467-022-33890-w PubMed 36253467
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