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pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T
(Plasmid #225062)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225062 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CylLL-S15T
  • Species
    E. faecalis
  • Insert Size (bp)
    3765
  • Mutation
    S15T
  • Promoter CMV
  • Tag / Fusion Protein
    • Multiple tags due to polycistronic expression (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

also encodes CylM

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T was a gift from Wilfred van der Donk (Addgene plasmid # 225062 ; http://n2t.net/addgene:225062 ; RRID:Addgene_225062)

  • For your References section:

    Expression and Subcellular Localization of Lanthipeptides in Human Cells. Eslami SM, Padhi C, Rahman IR, van der Donk WA. ACS Synth Biol. 2024 Jul 19;13(7):2128-2140. doi: 10.1021/acssynbio.4c00178. Epub 2024 Jun 26. 10.1021/acssynbio.4c00178 PubMed 38925629
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